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| Enrione,Javier; Osorio,Fernando; López,Daniel; Weinstein-Oppenheimer,Caroline; Fuentes,Miguel A; Ceriani,Ricardo; Brown,Donald I; Albornoz,Fernando; Sánchez,Elizabeth; Villalobos,Patricio; Somoza,Rodrigo A; Young,Manuel E; Acevedo,Cristian A. |
| Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30ºC could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin,... |
| Tipo: Journal article |
Palavras-chave: Chitosan; Fibrin; Gelatin; Hyaluronic acid; Scaffold. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500020 |
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| Castillo,Viviana M; Brown,Donald I. |
| Echinolittorina peruviana (Lamarck, 1822), a gonochoric representative of the Littorinidae on the SE Pacific coast, has a male reproductive system adapted for internal fertilization. We describe this system at both macro- and microscopic levéis, particularly the compartmentalized organization of the gonad, and the morphology of the penis. The male reproductive system has a variegated conical gonad-digestive gland complex. The gonad presents three compartments, 1) gametogenic acinar among the glandular acini, 2) periacinar with a layer of fusiform somatic cells and, 3) interacinar with glycogen storage cells shared with glandular acini. Spermatogenesis occurs within the acinar gametogenic compartment, with the germinal line organized in centripetal form... |
| Tipo: Journal article |
Palavras-chave: Mollusca; Microscopic anatomy; Male reproductive system; Littorinidae. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000200030 |
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| Collado,Gonzalo A; Brown,Donald I. |
| This study describes the microscopic anatomy of the reproductive systems of the keyhole limpets Fissurella latimarginata and Fissurella cumingi to test the existence of a possible isolating reproductive mechanism of morphophysiological type between species, and consequently to determine if the findings provide a valid taxonomic characteristic to distinguish these sympatric species. The results show that in both species the reproductive system is intimately associated with the excretory system. The gonad has a septa organization, and is connected with the distal portion of the right kidney by means of a renopericardial duct which functions as a genital duct. The gametes are evacuated to the mantle cavity through the right kidney opening. Besides transport... |
| Tipo: Journal article |
Palavras-chave: Mollusk; Fissurellidae; Reproductive system; Microscopic anatomy; Taxonomy. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200012 |
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